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Creators/Authors contains: "James, Anthony"

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  1. We present KODIS, a dyadic dispute resolution corpus containing thousands of dialogues from over 75 countries. Motivated by a theoretical model of culture and conflict, participants engage in a typical customer service dispute designed by experts to evoke strong emotions and conflict. The corpus contains a rich set of dispositional, process, and outcome measures. The initial analysis supports theories of how anger expressions lead to escalatory spirals and highlights cultural differences in emotional expression. We make this corpus and data collection framework available to the community, 
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  2. Innovative tools are essential for advancing malaria control and depend on an understanding of molecular mechanisms governing transmission of malaria parasites by Anopheles mosquitoes. CRISPR/Cas9-based gene disruption is a powerful method to uncover underlying biology of vector-pathogen interactions and can itself form the basis of mosquito control strategies. However, embryo injection methods used to genetically manipulate mosquitoes (especially Anopheles ) are difficult and inefficient, particularly for non-specialist laboratories. Here, we adapted the ReMOT Control ( Re ceptor- m ediated O vary T ransduction of C argo) technique to deliver Cas9 ribonucleoprotein complex to adult mosquito ovaries, generating targeted and heritable mutations in the malaria vector Anopheles stephensi without injecting embryos. In Anopheles , ReMOT Control gene editing was as efficient as standard embryo injections. The application of ReMOT Control to Anopheles opens the power of CRISPR/Cas9 methods to malaria laboratories that lack the equipment or expertise to perform embryo injections and establishes the flexibility of ReMOT Control for diverse mosquito species. 
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